Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Gleditsia sinensis Lam. (Fabaceae) is an endemic species in China, which has a wide range of ecological functions and high economic value. G. belongs to androdioecy, the stamens perfect flowers are aborted, meaning that flower functional female flower. Understanding dynamic process flowering characteristics pollen morphology effectively determine viability vitality, suitable conditions for short-term storage can provide theoretical basis technical reference hybrid breeding germplasm conservation sinensis. In this study, male plants Guiyang area were used as research materials. The was recorded through field observation. observed analyzed with scanning electron microscope (SEM). germination studied vitro method, vitality also determined using four staining methods. effects different temperatures water contents on rate discussed. results showed had short, single period, lasting 2–3 days from opening shedding. artificially divided into five stages. Pollen grains oblate spheroidal, tricolporate equatorial elongated endoapertures sporoderm surface reticulate. MTT (Thiazolyl Blue Tetrazolium Bromide) method could accurately quickly highest 65.89% ± 3.41%, length tube 3.96 mm after cultured 15% sucrose + 100 mg/L boric acid 20 calcium chloride 24 h. It necessary collect at big bud stage, conducive improving efficiency collection because been mature stage. When it came preservation, significantly affected by time, temperature content. still being stored −80 °C 30 when moisture content decreased 9%, only 28.84% compared before storage. conclusion, study comprehensively systematically morphology, determination preservation methods sinensis, providing cross regional utilization resources.